Coding

Part:BBa_I761004:Design

Designed by: 2007 NYMU_Taiwan iGEM Team   Group: iGEM07_Taipei   (2007-10-19)


TAT signal+IDE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 765
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 765
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 765
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1374
    Illegal SapI.rc site found at 805
    Illegal SapI.rc site found at 973


Design Notes

  • To combine TAT signal and mouse IDE, we used two separate PCR reaction to amplify each DNA fragment. We design the reverse primer of TAT signal and the forward primer of mouse IDE to be complement to each other, so when we mixed the product of each PCR reaction together, than used the forward primer of TAT signal and the reverse primer of mouse IDE to conduct PCR reaction, we can isolate the combine DNA product of TAT signal and mouse IDE. (Method devised by Dr. Chang)
  • There is an internal XbaI site in the sequence. Therefore we use blunt end ligation to overcome this problem. Further clean up is need to make this part a true Biobrick.

Source

  • NCBI accession number: BC041675
  • K12 E. coli genomic DNA

References